M.G. El-Bardeicy**, Z. A. El-Sherif*, A.O. Mohamed*^a1 and M.F. El-Tarras **

* National Organization for Drug Control and Research (NODCAR), 6-Abu Hazem Street, Pyramids Ave., P.O.  Box 29, 35521, Giza, Egypt. *^a1  Present  occupation, Associate Prof. Umm Al- Qura University- Faculty of pharmacy - Department of pharmaceutical chemistry - Makkah- Saudi Arabia B.O Box 13174

**Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr EL-Ainy Street, Cairo 11562, Egypt.

a Corresponding author^, s email: Afaf_osmanelteti@yahoo.com

Summery A simple and sensitive derivative ratio spectrophotometric method is described for analysis of omeprazole (Ome), lansoprazole (Lan) and pantoprazole sodium (Pan). The method involves the reaction of these drugs with alkaline potassium permanganate, where a manganate ion is produced. The reaction is monitored spectrophotometrically by measuring the amplitude of the first derivative of the ratio spectra of reaction product at 584, 453 and 403 nm corresponding to zero crossing of the blank reagent. The calibration graphs were linear over the concentration range     11-110 mg ml^-1, 12.5–100 mg ml^-1 and 13–130 mg ml^-1 for Lan, Ome and Pan, respectively. The proposed method was applied successfully for the determination of these drugs in pure forms and in pharmaceutical dosage forms. The obtained results compared statistically with those of the official or reported methods for the analysis of these drugs, reveled high accuracy and good precision. The validity of the method was assessed according to USP guidelines and also by applying standard addition technique.