Determination of some Cardiovascular Drugs through Condensation with p-Dimethylaminocinnamaldehyde

Laila El Sayed Abdel Fattah.1 Elham Anwer Taha.2 and Taghreed Abdel Fattah Mohamed.2*

1-Analytical Chemistry Department, Faculty of Pharmacy. Cairo University, Kasr el Aini Street, 11562 Cairo, Egypt.

2-National Organization for Drug Control and Research (NODCAR), 6 abou Hazem Pyramids Avenue P.O. BO X29. Giza, Egypt.

* E-mail; taghreednodcar@yahoo.com

 

Summary: This work presents simple, sensitive and validated spectrophotometric method for the determination of some cardiovascular drugs namely Amlodipine besylate (AM), dobutamine HCl (DO), and carvedilol (CA) in their drug substances and drug products.  The method depends on  condensation of the primary or secondary amino group with p-dimethylaminocinnamaldehyde (p-DAC) in methanol to form colored products measured at 472, 484, and 488 nm for AM, DO, and CA respectively.   Under the optimum reaction conditions, good linearity were obtained in the concentration range of 1.0-20.0, 0.5-8.0, and 1.0-12.0 µg ml-1 for the three mentioned drugs respectively. The method was validated according to ICH guidelines and the results were statistically compared with the official methods.  The limit of detection (LOD) and limit of quantification (LOQ) were calculated. Apparent molar absorbtivity, sensitivity index, stability constant (Kf), and free energy change (ΔG) of the reaction products were also calculated. 
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Sabrein Ibrahim,
Oct 12, 2016, 12:53 PM
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